研究方向简介：
长期从事膜蛋白结构与功能的核磁共振研究，重点围绕线粒体膜蛋白、离子通道及受体蛋白等重要生物大分子体系，发展并应用液体核磁共振（NMR）技术，在近生理膜环境下解析膜蛋白的结构特征、动态行为及其分子作用机制。研究中整合冷冻电镜（Cryo-EM）、单分子膜片钳电生理、细胞功能实验及生物化学等多学科技术手段，系统揭示膜蛋白转运、信号转导及小分子调控机制，为相关疾病机制研究和药物靶标发现提供结构生物学依据。此外，还开展液体NMR在肿瘤代谢组分分析及食品成分定量检测等领域的应用研究，为核磁共振技术在生命科学与食品科学中的交叉应用提供了新的研究思路和技术支撑。
主持国家自然科学基金青年基金项目一项及中国科学院合肥物质院院长基金青年“火花”项目一项。围绕线粒体蛋白转运系统、丙酮酸转运载体（MPC）、成纤维生长因子受体1（FGFR1）近膜区等开展了系列研究，在膜蛋白结构解析及蛋白-膜相互作用机制方面取得重要进展。相关成果发表于《Cell Research》《Nature Communications》《International Journal of Molecular Sciences》《Biomolecules》等期刊。
发表SCI论文
1. Yong Liu, Jing Yang, Maosen Ruan, Huiqin Zhang, Junfeng Wang, Yunyan Li, NMR-Based Characterization of the Interaction between Yeast Oxa1-CTD and Ribosomes, Int. J. Mol. Sci., 2023, 24(19): 14657 (共同通讯作者)
2. Zhen Wang, Wen Ding, Maosen Ruan, Yong Liu, Jing Yang, Huiqin Zhang, Bing Shen, Junfeng Wang, Yunyan Li, NMR and Patch-Clamp Characterization of Yeast Mitochondrial Pyruvate Carrier Complexes, Biomolecules, 2023, 13(5): 719 (共同通讯作者)
3. Yunyan Li, Yong Liu, Huiqin Zhang, Zhen Wang, Maosen Ruan, Jiarong Wang, Jing Yang, Bo Wu, Junfeng Wang, NMR and biochemical characterization of the interaction between FGFR1 juxtamembrane domain and phospholipids, Magn. Reson. Lett., 2022, 2, 204-212 (第一作者)
4. Shu Zhou, Maosen Ruan, Yunyan Li, Jing Yang, Suwen Bai, Christian Richter, Harald Schwalbe, Can Xie, Bing Shen, Junfeng Wang, Solution structure of the voltage-gated Tim23 channel in complex with a mitochondrial presequence peptide, Cell Res., 2021, 31, 821–824
5. Yunyan Li, Wenping Mao, Chaoyang Liu, Xu Zhang, Junfeng Wang, Quantitative Determination of Fatty Acid Compositions in Edible Oils Using J-Selective 13C QDEPT, Food Anal. Methods, 2019, 12 (4), 991-997 (第一作者)
6. Xiaoyu Ma*, Lin Wang*, De Huang*, Yunyan Li*, Dongdong Yang, Tingting Li, Fudong Li, Linchong Sun, Haoran Wei, Kun He, Fazhi Yu, Debiao Zhao, Lan Hu, Songge Xing, Zhaoji Liu, Kui Li, Jing Guo, Zhenye Yang, Xin Pan, Ailing Li, Yunyu Shi, Junfeng Wang, Ping Gao, Huafeng Zhang, Polo-like kinase 1 coordinates biosynthesis during cell cycle progression by directly activating pentose phosphate pathway, Nat. Commun., 2017, 8 (1), 1506 (共同第一作者)
7. Chanhao Wang, Yunyan Li, Yanqin Lin, Yonghua Wang, Zhong Chen, Lei Zhu, Junfeng Wang, In situ enzymatic hydrolysis characterisation of phospholipid using 1H NMR in a heterogeneous environment, LWT - Food Sci. Technol., 2023, 177, 114592
8. Yu Wang, Hongze Hu, Siming Yuan, Yunyan Li, Kaiming Cao, Hongbin Sun, Yangzhong Liu, Cuprous ions can disrupt the structure and functions of the RING finger domain of RNF11, Inorg. Chem. Front., 2022, 9, 3820-3827
9. Sajid ur Rehmana, ShuaiXu, Huangtao Xu, Tongxiang Tao, Yunyan Li, Zhiwu Yu, Kun Ma, Weihong Xu, Junfeng Wang, The Role of NMR in Metal Organic Frameworks: Deep Insights into Dynamics, Structure and Mapping of Functional Groups, Mater. Today Adv., 2022, 16, 100287
10. Xuekun Fu, Yunyan Li, Tongling Huang, Zhiwu Yu, Kun Ma, Meng Yang, Qingli Liu, Haobo Pan, Huaiyu Wang, Junfeng Wang, Min Guan, Runx2/Osterix and Zinc Uptake Synergize to Orchestrate Osteogenic Differentiation and Citrate Containing Bone Apatite Formation, Adv. Sci., 2018, 5 (4), 1700755